Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K18me2 antibody at 0.5 ug/ml showed a band of Histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.
A peptide dot blot shows the recombinant H3K18me2 antibody only reacts to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with unmodified (K18Ctrl) or monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2) or dimethylated Lysine 36 (K36me2).
The recombinant H3K18me2 antibody specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.
Supplier Page from NSJ Bioreagents for Recombinant H3K18me2 Antibody / Dimethyl Histone H3 Lysine 18 [Recombinant Rabbit Monoclonal]